Cross-contamination and misidentification of cell lines are unfortunately very common within the research community. Many contaminated cell lines were overgrown by the contaminating cell line during establishment and so authentic stocks probably do not exist; in those cases all work has been performed on the

contaminant, which may come from a different species, tissue or cell type.

To let researcher check the true identity of their cell lines to have a peace of mind- a requirement increasingly imposed by scientific journals and funding agencies - BioMaxLab offers a cell line authentication service.

General recommendations are to authenticate:

1. When a new line is established or acquired to determine an identity for the cell line.

2. Before freezing.

3. When the culture is actively growing for 2-3 months.

4. When the performance of the cell line is not consistent or results are unexpected.

We offer 2 levels of service, Basic Service and Basic Service with Profile Search.

The Basic Service includes a authentication report for each line submitted that is suitable for proof of cell line authentification which may be required by some journals and grant funding agencies. The results are used to check for cross contamination, cell line mix-ups and percent match between lines or to establish the identity of a new cell line.

Basic Service with Profile Search includes the above, together with cross referencing against repositories ATCC, DSMZ, and JCRB databases to check the authenticity of your cell line.

Service Provided

We provide STR profiling analysis for cell lines of human origin by showing different numbers of markers.

AUTH10: GenePrint 10 System (Promega), Markers included in this analysis are amelogenin, CSF1PO, D13S317, D16S539, D21S11, D5S818, D7S820, THO1, TPOX, and vWA.

Or

AUTH18: PowerPlex 18D (Promega), Markers included in the kit are amelogenin, CSF1PO, D13S317, D16S539, D21S11, D5S818, D7S820, THO1, TPOX, vWA, D18S51, D3S1358, FGA, D8S1179, Penta E, Penta D, D2S1338 and D19S433.